Regulatory

Part:BBa_K112406:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-22)


cadC promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 147
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Biobricking of cadC promoter and cadC gene

PCR cadC-F and cadC-R on MG1655 	   (2336 bp, EcoR1/BamH1)

Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)

Product is pBca1256-K112406

----------------------------
cb10013-cadC-F                 cgataGAATTCatgAGATCTcaccagcaatttgcgggcatatc
cb10012-cad-R                 cgtagGGATCCtgaccagaccaaactggcg

References